Tg(APPSwe,tauP301L)1Lfa
Transgene Detail

Summary

• Symbol: Tg(APPSwe,tauP301L)1Lfa
• Name: transgene insertion 1, Frank M LaFerla
• MGI ID: MGI:2672831
• Synonyms: Tg(APP*Swe,MAPT*P301L)1Lfa
• Transgene: Tg(APPSwe,tauP301L)1Lfa Location: Chr2:87692807-87692810 bp Genetic Position: Chr2, Syntenic
• Alliance: Tg(APPSwe,tauP301L)1Lfa page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intergenic deletion
• Tg(APPSwe,tauP301L)1Lfa expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  human

  MAPT (4137)

  Mapt	(MGI:97180)

• Mutation details: To develop a model of Alzheimer's Disease, mice harboring mutant human APP (Swedish double mutation; K670N, M671L) and MAPT (P301L) as well as Psen^tm1Mpm were generated by microinjection of the APP and MAPT transgenic constructs into single cell embryos harvested from mice homozygous for Psen1^tm1Mpm. Southern blot analysis indicated that both transgenic constructs integrated into the same site. Western blot analysis showed APP and MAPT levels to be ~4 fold higher in hemizygous mice and ~6 (APP) to ~7 (Mapt) fold higher homozygous mice, relative to non transgenic mice. Amyloid-Beta peptide (both 40 and 42) was detected in transgenic mice, with greater levels in homozygous mice than in hemizygous mice. Expression was confined to the CNS. Highest steady state levels of proteins were detected in Alzheimer's Disease related regions including the hippocampus and cerebral cortex. Transgenic protein was not detected in the cerebellum. The transgene inserted on Chr 2 causing a 3 bp deletion. (J:84847)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 3 lines available

References

• Original: J:84847 Oddo S, et al., Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Abeta and synaptic dysfunction. Neuron. 2003 Jul 31;39(3):409-21
• All: 733 reference(s)