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Tg(BCL2)36Wehi
Transgene Detail
Summary
Symbol: Tg(BCL2)36Wehi
Name: transgene insertion 36, Walter and Eliza Hall Institute of Medical Research
MGI ID: MGI:3052954
Synonyms: Bcl-2-transgenic, E mu-bcl-2-36, Emu-Bcl-2-36
Transgene: Tg(BCL2)36Wehi  Location: unknown  
Alliance: Tg(BCL2)36Wehi page
Transgene
origin
Strain of Origin:  (C57BL/6JWehi x SJL/JWehi)F2
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(BCL2)36Wehi expresses 1 gene
 
Mutation detailsThe transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. (J:93111)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying Tg(BCL2)36Wehi
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Notes

The transgene is expressed in both T cell and B cell lineages.

On a C57BL/6 background hemizygotes show increased numbers of B lymphocytes, Ig-secreting cells and serum Ig, as well as a heightened and prolonged antibody response to immunization. T-cells withstand prolonged culture in the absence of growth factors and are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells, or Fas ligand. These hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization, and serve as a robust source for the production of B-cells, antibodies T-cell lines or hybridomas.

Although transgenic mice on a (C57BL/6 x SJL)F2 background exhibit a mild increase in spontaneous lymphoma and plasmacytoma occurrence (<10% to 18 months), on the BALB/c and C57BL/6 backgrounds tumor incidence is insignificant. Hemizygotes on a background involving C57BL/6 and SJL (but not on the C57BL/6 background) develop an autoimmune disease characterized by immune complex glomerulonephritis, anti-nuclear antibodies, lymphadenopathy, and myocardial infarction.

References
Original:  J:93111 Strasser A, et al., Abnormalities of the immune system induced by dysregulated bcl-2 expression in transgenic mice. Curr Top Microbiol Immunol. 1990;166:175-81
All:  53 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory