Tg(CAG-EGFP)D4Nagy
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP)D4Nagy
• Name: transgene insertion D4, Andras Nagy
• MGI ID: MGI:3055027
• Synonyms: D4/XEGFP, Tg(ACTB-EGFP)D4Nagy, Tg(CAG-EGFP)D4Nagy, Tg(CMV-GFP)1Jae, Tg(CMV-GFP)1Nagy, Tg(GFPX)4Nagy, X-GFP, X^GFP, XGFP, Xp-GFP
• Transgene: Tg(CAG-EGFP)D4Nagy Location: unknown Genetic Position: ChrX, Syntenic
• Alliance: Tg(CAG-EGFP)D4Nagy page

Transgene origin

• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains the enhanced Green Fluorescent Protein driven by chicken beta-actin promoter and CMV intermediate early enhancer. Transgenic mice were generated by embryonic stem (ES) cell-mediated transgenesis. The construct was electroporated into R1 ES cells and random integration occurred on the X chromosome just distal of Timp and Syn1. (J:69995)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Transgene expression is seen as fluorescence under exposure to a 488 nm light source. In transgenic mice the reporter may be detected as early as ~E2.75, as morula stage embryos begin to compact. Expression of GFP is spatiotemporally ubiquitous, though levels differ between different lineages (e.g., expression is slightly higher in the heart).

No discernible phenotype, other than green fluorescence, has been observed in any mice carrying this transgene.

References

• Original: J:69995 Hadjantonakis AK, et al., Non-invasive sexing of preimplantation stage mammalian embryos. Nat Genet. 1998 Jul;19(3):220-2
• All: 48 reference(s)