Tg(PF4MER)6Kra
Transgene Detail

Summary

• Symbol: Tg(PF4MER)6Kra
• Name: transgene insertion 6, Katya Ravid
• MGI ID: MGI:3054406
• Synonyms: PF4MERGH
• Transgene: Tg(PF4MER)6Kra Location: unknown
• Alliance: Tg(PF4MER)6Kra page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(PF4MER)6Kra expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MYC (4609)

  Bmyc	(MGI:88184)
  Myc	(MGI:97250)

• Mutation details: The transgene is a 3.9-kb fragment which contains the rat platelet factor 4 (Pf4) promoter followed by exons 2 and 3 of human myc fused to the human estrogen receptor (MER), followed by a 442-base pair region of the HGH poly(A) tail starting from the stop codon of the HGH gene. Two copies of the transgene integrated in this line. Expression of the transgene was detected in bone marrow megakaryocytes. (J:35507)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Mice homozygous for this transgene on an FVB/N background are viable and fertile. Expression of the transgene is exclusively in platelets and megakaryocytes in female carrier mice or in male carrier mice given injections of estrogen. Megakaryopoiesis is increased and they exhibit essential thrombocythemia. This strain may serve as a model for a myeloproliferative disorder resembling essential thrombocythemia.

References

• Original: J:35507 Thompson A, et al., Deregulated expression of c-myc in megakaryocytes of transgenic mice increases megakaryopoiesis and decreases polyploidization. J Biol Chem. 1996 Sep 20;271(38):22976-82
• All: 1 reference(s)