Tg(APP)8.9Btla
Transgene Detail

Summary

• Symbol: Tg(APP)8.9Btla
• Name: transgene insertion 8.9, Bruce Lamb
• MGI ID: MGI:2447591
• Synonyms: APPYAC, hABetaPP, hAPP-YAC, Py8.9, Tg(APP)C9.24Jgh
• Transgene: Tg(APP)8.9Btla Location: unknown
• Alliance: Tg(APP)8.9Btla page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:80947
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(APP)8.9Btla expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

• Mutation details: A 650 kb YAC containing the entire human APP gene and 350 kb of flanking sequence was randomly integerated into ES cells. Southern blot analysis confirmed that construct rearrangement did not occur and that the animals carried a single copy of the intact transgene. Quantitative RT-PCR analysis of transgenic and wild-type mice revealed that the levels of transgenic human alternate transcripts corresponded with those of the endogenous alternative transcripts. The human APP promoter drives expression in the brain, heart, kidney and testes. (J:80947)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:80947 Lamb BT, et al., Introduction and expression of the 400 kilobase amyloid precursor protein gene in transgenic mice [corrected]. Nat Genet. 1993 Sep;5(1):22-30
• All: 16 reference(s)