Tg(TcrHEL3A9)1Mmd
Transgene Detail

Summary

• Symbol: Tg(TcrHEL3A9)1Mmd
• Name: transgene insertion 1, Mark M Davis
• MGI ID: MGI:2385683
• Synonyms: 3A9 anti-HEL, 3A9 TCR, Tg(TcrHEL3A9)Mmd
• Transgene: Tg(TcrHEL3A9)1Mmd Location: unknown
• Alliance: Tg(TcrHEL3A9)1Mmd page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcrHEL3A9)1Mmd expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Transgenic mice produced from this construct carry transgenes encoding the T cell receptor alpha and beta (Tcra and Tcrb, respectively) chains from the hen egg lysozyme (HEL)-specific T cell hybridoma 3A9. Two vectors were constructed and subsequently co-injected into C57BL/6J eggs. The first vector contained the Tcra variable and joining regions (V and J, respectively) and a 3.5kb segment 3' to the Tcra constant region. The second vector contained the Tcrb variable, diversity, and joining regions (V, D, and J, respectively) and a 7.5kb segment 3' to the Tcrb constant region, which included the Tcrb enhancer region. Transgenic mice expressed the alpha and beta chains of a T cell receptor specific for hen egg lysozyme. Two female founders were generated. (J:73608)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available

References

• Original: J:73608 Ho WY, et al., Resting and anergic B cells are defective in CD28-dependent costimulation of naive CD4+ T cells. J Exp Med. 1994 May 1;179(5):1539-49
• All: 58 reference(s)