Tg(APPswe,PSEN1dE9)85Dbo
Transgene Detail

Summary

• Symbol: Tg(APPswe,PSEN1dE9)85Dbo
• Name: transgene insertion 85, David R Borchelt
• MGI ID: MGI:3524957
• Synonyms: 2xTg Ad, APdE9, APP-PS1, APP/PS1, APPswe/PS1dE9, APPswe/PS1deltaE9, Mo/Hu APPswe PS1dE9
• Transgene: Tg(APPswe,PSEN1dE9)85Dbo Location: Chr9:112832728-112832729 bp Genetic Position: Chr9, Syntenic
• Alliance: Tg(APPswe,PSEN1dE9)85Dbo page

Beta-amyloid plaques in Tg(APPswe,PSEN1dE9)85Dbo/0 mice and neurofibrillary tangles in Mapt^tm1(EGFP)Klt/Mapt^tm1(EGFP)Klt Tg(MAPT)8cPdav/0 mice

Show the 1 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutations: Duplication, Insertion
• Tg(APPswe,PSEN1dE9)85Dbo expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  mouse

  App (MGI:88059)

  The mouse gene was humanized in the A-beta domain and modified to encode the Swedish mutations K595N/M596L.

  human

  PSEN1 (5663)

  Psen1

  (MGI:1202717)

  Exon 9 is deleted.

• Mutation details: Two transgenes inserted at a single locus in Chromosome 9 between Arpp21 and Pdcd6ip. Each transgene is controlled by the mouse prion promoter and contains a cDNA sequence. In one transgene the cDNA encodes a chimeric amyloid beta (A4) precursor protein (APPswe). In the second transgene the cDNA encodes the "DeltaE9" mutation of human presenilin 1. The DeltaE9 mutation of the human presenilin 1 gene is a deletion of exon 9 and corresponds to a form of early-onset Alzheimer's disease. The amyloid beta precursor protein coding sequences were altered by replacing mouse sequence encoding three amino acids of the A-beta domain with the human coding sequence for these residues. The chimeric amyloid beta (A4) precursor protein sequence was then further modified to encode the Swedish mutations K595N/M596L found in human. Both the transgenic peptide and holoprotein are detected by Signet Laboratories' monoclonal 6E10 antibody, which is specific for human sequence within this region. Human presenilin protein, which in high levels displaces detectable endogenous mouse protein, is immunodetected in the double transgenic mouse in whole brain protein homogenates. Human amyloid precursor protein is also immunodetected in these mice in whole brain protein homogenates. Transgene insertion occurred on Chr 9, causing a 1 bp duplication. (J:78664, J:227541)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 13 lines available

Notes

Mice carrying this double transgene develop beta-amyloid deposits in the brain by 6 to 7 months of age.

References

• Original: J:78664 Jankowsky JL, et al., Co-expression of multiple transgenes in mouse CNS: a comparison of strategies. Biomol Eng. 2001 Jun;17(6):157-65
• All: 1245 reference(s)