Tg(Ins2-NP)25-3Olds
Transgene Detail

Summary

• Symbol: Tg(Ins2-NP)25-3Olds
• Name: transgene insertion 25-3, Michael BA Oldstone, MD
• MGI ID: MGI:3573697
• Synonyms: RIP-LCMV, RIP-LCMV NP, RIP NP 25-3
• Transgene: Tg(Ins2-NP)25-3Olds Location: unknown
• Alliance: Tg(Ins2-NP)25-3Olds page

Transgene origin

• Strain of Origin: C57BL/6 x BALB/c

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: This transgene encodes the nucleoprotein (NP) from Armstrong's clone of the lymphocytic choriomeningitis virus (LCMV) regulated by the rat insulin promoter. Upstream of the NP cDNA are 660 base pairs of regulatory elements in addition to the rat insulin promoter. Downstream of the NP c-DNA is the SV40 small T-antigen intron and late polyadenylation signal. A stable transcript of the expected size was present in the pancreas, as determined by Northern blot analysis. RT-PCR analysis demonstrated that the transcript was expressed in pancreas and thymus, but not the spleen, brain, liver, kidney, heart, muscle or lung. In a T cell recognition assay, LCMV-specific cytotoxic T lymphocytes were shown to migrate specifically to the islets of Langerhans of transgenic mice, but not in control mice. (J:81284, J:81287)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:81284 Oldstone MB, et al., Virus infection triggers insulin-dependent diabetes mellitus in a transgenic model: role of anti-self (virus) immune response. Cell. 1991 Apr 19;65(2):319-31
• All: 20 reference(s)