Tg(Th-SNCA)5Eric
Transgene Detail

Summary

• Symbol: Tg(Th-SNCA)5Eric
• Name: transgene insertion 5, Eric K Richfield
• MGI ID: MGI:3530029
• Synonyms: hwalpha-SYN-5, Tg(Th-SNCA)5Fed, THsynWT
• Transgene: Tg(Th-SNCA)5Eric Location: unknown
• Alliance: Tg(Th-SNCA)5Eric page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Th-SNCA)5Eric expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SNCA (6622)

  Snca	(MGI:1277151)

• Tg(Th-SNCA)5Eric expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Th (25085)

  Th	(MGI:98735)

• Mutation details: The transgene contains a 9 kb rat tyrosine hydroxylase promoter, a splice donor/intron/splice acceptor, a poly(A) site from the human growth hormone gene, and a wild-type human alpha synuclein cDNA. RT-PCR detected transgene expression in midbrain, eye, and adrenal gland. Real time quantitative RT-PCR as well as in situ hybridization histochemistry detected high levels of transgene expression in the midbrain. Catecholaminergic nuclei in the brain of transgenic mice expressed both human mRNA and human protein. These mice express high levels of the human protein in the cell bodies of dopaminergic neurons in the midbrain and its main projection region, the striatum, dopaminergic dendrites, and nigrostriatal axons and terminals. (J:76482)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Line 88 was also generated using this wild-type construct.

References

• Original: J:76482 Richfield EK, et al., Behavioral and neurochemical effects of wild-type and mutated human alpha-synuclein in transgenic mice. Exp Neurol. 2002 May;175(1):35-48
• All: 8 reference(s)