Tg(Ins2-TFRC/OVA)296Wehi
Transgene Detail

Summary

• Symbol: Tg(Ins2-TFRC/OVA)296Wehi
• Name: transgene insertion 296, Walter and Eliza Hall Institute of Medical Research
• MGI ID: MGI:3578729
• Synonyms: RIP-mOVA, RIPmOVA
• Transgene: Tg(Ins2-TFRC/OVA)296Wehi Location: unknown
• Alliance: Tg(Ins2-TFRC/OVA)296Wehi page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-TFRC/OVA)296Wehi expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  chicken

  OVAL (396058)

  human

  TFRC (7037)

  Tfrc	(MGI:98822)

• Tg(Ins2-TFRC/OVA)296Wehi expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgene encodes a fusion protein comprising the carboxy-terminal 118 amino acids of the human transferrin receptor, which include the cytoplasmic tail, membrane-targeting signal and anchor domain of the protein, joined to amino acids 139-385 of mature ovalbumin. Its expression is directed by the rat insulin 2 promoter. (J:98589)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Ovalbumin (OV) is present at high levels in pancreatic beta cells and in kidney proximal tubule cells and at very low levels in testes of transgenic mice. No OV has been detected immunohistologically in any of multiple other tissues examined. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an OV-specific T cell receptor expressed on CD8+ T lymphocytes, exhibit thymic deletion of OT-1 T cells, indicating presence of OV at very low levels in the thymus. Western blot analysis reproducibly measured the pancreatic islet OV content as 2.2 ng/ug of protein.

References

• Original: J:98589 Kurts C, et al., Constitutive class I-restricted exogenous presentation of self antigens in vivo. J Exp Med. 1996 Sep 1;184(3):923-30
• All: 120 reference(s)