Tg(TcraCl4,TcrbCl4)1Shrm
Transgene Detail

Summary

• Symbol: Tg(TcraCl4,TcrbCl4)1Shrm
• Name: transgene insertion 1, Linda Sherman
• MGI ID: MGI:3586582
• Synonyms: C4, CL-4, CL4, CL-4 TCR, CL4-TCR, Clone 4, clone 4 TCR, Clone-4 TCR, (HA)-TCR
• Transgene: Tg(TcraCl4,TcrbCl4)1Shrm Location: unknown
• Alliance: Tg(TcraCl4,TcrbCl4)1Shrm page

Transgene origin

• Strain of Origin: (C57BL/6 x BALB/c)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraCl4,TcrbCl4)1Shrm expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc¹ H2^d H2-t18^c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer E_H, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, E_H, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2k^d. (J:90982, J:99756)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Gt(ROSA)26Sor^tm1(HA)Libl/Gt(ROSA)26Sor^tm1(HA)Libl Mog^tm1(cre)Gkl/? Tg(TcraCl4,TcrbCl4)1Shrm/0	involves: 129/Sv * BALB/c * C57BL/6
cx2	Tg(Ins2-HA)165Bri/? Tg(TcraCl4,TcrbCl4)1Shrm/?	involves: BALB/c * C57BL/6 * C57BL/10
cx3	Tg(Ins2-HA)165Bri/0 Tg(TcraCl4,TcrbCl4)1Shrm/0	involves: BALB/c * C57BL/6 * C57BL/10 * DBA/2
tg4	Tg(TcraCl4,TcrbCl4)1Shrm/?	B10.Cg-H2^d Tg(TcraCl4,TcrbCl4)1Shrm

Phenotypes:

Affected Systems	cn1	cx2	cx3	tg4
show or hide all annotated terms
behavior/neurological	√
impaired righting response	√
tremors	√
decreased locomotor activity	√
endocrine/exocrine glands		√	√
abnormal pancreatic islet morphology			√
insulitis		√	√
growth/size/body	√
weight loss	√
hematopoietic system				√
abnormal CD8-positive, alpha-beta T cell differentiation				√
abnormal T cell subpopulation ratio				√
homeostasis/metabolism		√	√
hyperglycemia		√	√
immune system	√	√	√	√
insulitis		√	√
abnormal CD8-positive, alpha-beta T cell differentiation				√
abnormal T cell subpopulation ratio				√
brain inflammation	√
spinal cord inflammation	√
optic nerve inflammation	√
mortality/aging		√	√
postnatal lethality			√
postnatal lethality, complete penetrance		√
nervous system	√
brain inflammation	√
spinal cord inflammation	√
optic nerve inflammation	√
abnormal oligodendrocyte morphology	√
abnormal optic nerve morphology	√
demyelination	√
vision/eye	√
abnormal optic nerve morphology	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:99756 Morgan DJ, et al., CD8(+) T cell-mediated spontaneous diabetes in neonatal mice. J Immunol. 1996 Aug 1;157(3):978-83
• All: 86 reference(s)