Tg(H2-Ea-Ins2)1Wehi
Transgene Detail

Summary

• Symbol: Tg(H2-Ea-Ins2)1Wehi
• Name: transgene insertion 1, William and Eliza Hall Institute
• MGI ID: MGI:3590216
• Transgene: Tg(H2-Ea-Ins2)1Wehi Location: unknown
• Alliance: Tg(H2-Ea-Ins2)1Wehi page

Transgene origin

• Strain of Origin: NOD/ShiLt

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(H2-Ea-Ins2)1Wehi expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ins2 (MGI:96573)

• Mutation details: The transgene comprises nucleotides -1,903 through -39 of the 5' flanking sequence of the MHC Class II gene H2-Ea (k allele), containing the promoter, followed by a 909 base pair mouse (NOD strain) genomic DNA fragment containing the sequence encoding prepronsulin II and the SV40 small t intron and polyadenylation signal.

  Transgene-derived mRNA is detectable in spleen and thymus of transgenic NOD/LtWehi mice, but not of control littermates, by reverse transcription followed by PCR (RT/PCR) using either primers for H2-Ea^k and proinsulin or two proinsulin primers. Immunoreactive proinsulin is detectable in medium from cultured transgenic splenocytes only after 8 days' culture and at levels 10^-2 - 10^-3 times those of cultured islets or islet cells; levels are increased by culture with interferon gamma and tumor necrosis factor alpha. (J:100232)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two lines of mice were created with this trasgenic construct on the NOD background. No distinction was made between them.

References

• Original: J:100232 French MB, et al., Transgenic expression of mouse proinsulin II prevents diabetes in nonobese diabetic mice. Diabetes. 1997 Jan;46(1):34-9
• All: 8 reference(s)