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Tg(Ins2-GAD2)2Lt
Transgene Detail
Summary
Symbol: Tg(Ins2-GAD2)2Lt
Name: transgene insertion 2, Edward H Leiter
MGI ID: MGI:3603349
Synonyms: TgN(RIP7hGAD65)Lt4 A-line, TgN(RIP7hGAD65)Lt4 (A line)
Transgene: Tg(Ins2-GAD2)2Lt  Location: unknown  Genetic Position: Chr15, Syntenic
Alliance: Tg(Ins2-GAD2)2Lt page
Transgene
origin
Strain of Origin:  NOD/ShiLt
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Ins2-GAD2)2Lt expresses 1 gene
 
Tg(Ins2-GAD2)2Lt expression driven by 1 gene
 
Mutation detailsThe transgene comprises the cDNA sequence encoding human glutamic acid decarboxylase 2 under control of a modified rat insulin 2 promoter composed of 9.5 kb of genomic DNA from upstream of the transcription initiation site fused to the first intron of Ins2. Densitrometric Southern blot analysis indicates presence of 4-5 copies of the transgene. FISH analysis and pseudo-Giemsa metaphase spread analysis showed integration to have occurred near the centromere of Chr 15. Expression in the pancreatic islets of transgenic mRNA has been demonstrated by reverse transcription followed by PCR (RT-PCR), and of GAD2 protein by western blot analysis. (J:100251)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
References
Original:  J:100251 Bridgett M, et al., Differential protection in two transgenic lines of NOD/Lt mice hyperexpressing the autoantigen GAD65 in pancreatic beta-cells. Diabetes. 1998 Dec;47(12):1848-56
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory