Tg(TcraBDC2.5,TcrbBDC2.5)1Doi
Transgene Detail

Summary

• Symbol: Tg(TcraBDC2.5,TcrbBDC2.5)1Doi
• Name: transgene insertion 1, Christophe Benoist
• MGI ID: MGI:2181203
• Synonyms: BDC2.5, BDC-2.5/N TCR Tg, Tg(TcraBDC2.5)2Doi, Tg(TcrbBDC2.5)2Doi
• Transgene: Tg(TcraBDC2.5,TcrbBDC2.5)1Doi Location: unknown
• Alliance: Tg(TcraBDC2.5,TcrbBDC2.5)1Doi page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraBDC2.5,TcrbBDC2.5)1Doi expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: This transgene results from the coinjection of two constructs derived from diabetogenic T-cell clone BDC2.5. The Tcra construct contains a rearranged Tcra sequence (V alpha J alpha) that begins 20 bp 5' of the ATG site and ends 30 bp 3' of the splice donor sequence of J alpha 17. The 6.5 Tcrb construct contains rearranged Tcrb sequence (V beta D beta J beta) flanked by 2 kb of 5' regulatory sequences and 3.5 kb of 3' DNA containing the unrearranged J beta 1.3 to J beta 1.6 sequences, and precedes a 12 kb sequence containing the C beta 2 region. (J:77007)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:77007 Katz JD, et al., Following a diabetogenic T cell from genesis through pathogenesis. Cell. 1993 Sep 24;74(6):1089-100
• All: 149 reference(s)