Tg(TcraCl1,TcrbCl1)1Shrm
Transgene Detail

Summary

• Symbol: Tg(TcraCl1,TcrbCl1)1Shrm
• Name: transgene insertion 1, Linda Sherman
• MGI ID: MGI:3617607
• Synonyms: Clone-1 TCR
• Transgene: Tg(TcraCl1,TcrbCl1)1Shrm Location: unknown
• Alliance: Tg(TcraCl1,TcrbCl1)1Shrm page

Transgene origin

• Strain of Origin: B10.D2-Hc¹ H2^d H2-T18^c/nSnJ

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraCl1,TcrbCl1)1Shrm expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: The transgene encodes a low avidity T cell receptor (TCR) that recognizes the influenza virus hemagglutinin (HA) epitope HA518-526 in the context of the H2-K^d MHC class I molecule. The rearranged Tcra and Tcrb genes, which have joined Valpha1/Jalpha11 and Vbeta8.2/D/Jbeta2.4, respectively, were amplified from cytotoxic T lymphocyte (CTL) clone 1. Clone 1 was derived from a transgenic B10.D2 mouse that expressed influenza virus HA as a self-antigen in pancreatic beta cells, which resulted in production of lower-avidity TCRs than those made by wild-type B10.D2 mice after infection with influenza virus strain A/PR/8/32. (J:97745)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

CD8⁺ T cells bearing this transgene, when transferred to mice that develop pancreatic tumors expressing influenza virus HA, can be induced to exhibit both effector function and antitumor activity only if HA-responsive CD4⁺ T cells are co-transferred and the mice are subsequently infected with influenza virus. : J:97745

References

• Original: J:97745 Lyman MA, et al., The fate of low affinity tumor-specific CD8+ T cells in tumor-bearing mice. J Immunol. 2005 Mar 1;174(5):2563-72
• All: 4 reference(s)