Tg(HCRT-MJD)1Stak
Transgene Detail

Summary

• Symbol: Tg(HCRT-MJD)1Stak
• Name: transgene insertion 1, Takeshi Sakurai
• MGI ID: MGI:2387763
• Synonyms: orexin/ataxin-3, ORX/ATX-Tg
• Transgene: Tg(HCRT-MJD)1Stak Location: unknown
• Alliance: Tg(HCRT-MJD)1Stak page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/1)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(HCRT-MJD)1Stak expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATXN3 (4287)

  Atxn3

  (MGI:1099442)

• Mutation details: The transgenic construct contained a carboxy terminal MJD cDNA fragment derived from a patient with Machado-Joseph disease adjoined to a human HCRT promoter. The promoter region consisted of 3.2 kb of 5' upstream region in addition to the the noncoding exon 1. Expression was directed specifically in hypocretin neurons. The MJD fragment contained 77 polyglutamine repeats and was tagged with a Myc epitope tag for histological examination. Expression of the MJD fragment resulted in the ablation of hypocretin containing neurons (J:69620)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:69620 Hara J, et al., Genetic ablation of orexin neurons in mice results in narcolepsy, hypophagia, and obesity. Neuron. 2001 May;30(2):345-54
• All: 37 reference(s)