Tg(Myh6-EP300)39Csk
Transgene Detail

Summary

• Symbol: Tg(Myh6-EP300)39Csk
• Name: transgene insertion 39, Chugai Pharmaceutical Co, Ltd
• MGI ID: MGI:3640673
• Synonyms: line 39-TG, p300 TG, p300-TG
• Transgene: Tg(Myh6-EP300)39Csk Location: unknown
• Alliance: Tg(Myh6-EP300)39Csk page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-EP300)39Csk expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EP300 (2033)

  Ep300

  (MGI:1276116)

• Mutation details: A full-length cDNA encoding human E1A binding protein p300 was ligated to the 5' end of a 5.5-kb fragment of mouse genomic DNA that includes the entire, promoter-containing intergenic region between the beta- (downstream) and alpha-cardiac myosin heavy chain genes. RT-PCR with transgene-specific primers detected mRNA expression in heart, but not in lung, liver or kidney. Immunoblot analysis detected 8-9-fold as much of the (endogenous plus transgene-encoded) protein in cardiac nuclear extracts from transgenic than from wild-type mice. (J:110549)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The original publication (J:110549) does not name or distinguish among three lines derived from independent transgenic founders. Kawamura et al. (J:110550) identify lines 21 and 39.

References

• Original: J:110549 Yanazume T, et al., Cardiac p300 is involved in myocyte growth with decompensated heart failure. Mol Cell Biol. 2003 May;23(10):3593-606
• All: 4 reference(s)