Tg(APPSwLon)96Btla
Transgene Detail

Summary

• Symbol: Tg(APPSwLon)96Btla
• Name: transgene insertion 96, Bruce Lamb
• MGI ID: MGI:3693359
• Synonyms: APP^K670N/M671L and APP^V717Im, J1.96, K670N/M671L + V717I, Tg(APP*Swe*Lon)96Btla
• Transgene: Tg(APPSwLon)96Btla Location: unknown
• Alliance: Tg(APPSwLon)96Btla page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:42613
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(APPSwLon)96Btla expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  The gene encodes the Swedish and London mutations.

• Mutation details: A 650 kb YAC transgene containing the entire human amyloid beta (A4) precursor protein gene and approximately 250 kb of flanking sequence was altered to include the Familial Alzheimer's Disease- (FAD-) associated "Swedish" double mutation, APPK670N/M671L, and the "London" mutation, APPV717I. RT-PCR analysis shows hemizygous mice express the mutant human and endogenous mouse APP mRNA at similar levels in brain and peripheral tissues; levels of the most common alternative splice variants (encoding human APP-695, -751 and -770) parallel those of the endogenous mouse transcripts. Levels of total amyloid-beta and of longer amyloid-beta peptides (species terminating at amino acids 42/43) are higher than in wildtype mice, but not as high as in a similar strain carrying only the APPK670N/M671L "Swedish" mutant transgene. (J:42613)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

The transgene construct was injected into embryonic stem (ES) cells.

References

• Original: J:42613 Lamb BT, et al., Altered metabolism of familial Alzheimer's disease-linked amyloid precursor protein variants in yeast artificial chromosome transgenic mice. Hum Mol Genet. 1997 Sep;6(9):1535-41
• All: 2 reference(s)