Tg(Prnp-App/APPswe)E1-2Dbo
Transgene Detail

Summary

• Symbol: Tg(Prnp-App/APPswe)E1-2Dbo
• Name: transgene insertion E1-2, David R Borchelt
• MGI ID: MGI:3696473
• Synonyms: E1-2 line, line E1-2, Mo/Hu APPswe, Mo/Hu APPswe line E1-2, Tg(Prnp-App/APP*Swe)E1-2Dbo
• Transgene: Tg(Prnp-App/APPswe)E1-2Dbo Location: unknown
• Alliance: Tg(Prnp-App/APPswe)E1-2Dbo page

Transgene origin

• Strain of Origin: (C57BL/6J x C3H/HeJ)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp-App/APPswe)E1-2Dbo expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

• Mutation details: This transgene expresses a modified mouse amyloid precursor protein cDNA encoding the 695-amino acid isoform with a "humanized" Abeta domain amino acid sequence that includes the familial Alzheimer disease- (FAD-) associated Swedish double mutation (Lys595Asn and Met596Leu). The cDNA was inserted into an expression cassette containing the promoter, 5' intronic and 3' untranslated region sequences of the mouse prion protein gene. (J:80782)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three transgenic lines were generated and designated by the authors lines Q2-2, E1-2 and C3-3 (Tg(APP695)3Dbo). They were estimated, on the basis of Southern blot analysis, to carry 3-10 copies of the transgene.

References

• Original: J:80782 Borchelt DR, et al., A vector for expressing foreign genes in the brains and hearts of transgenic mice. Genet Anal. 1996 Dec;13(6):159-63
• All: 4 reference(s)