Tg(Prnp-ITM2B/APP695*40)1Emcg
Transgene Detail

Summary

• Symbol: Tg(Prnp-ITM2B/APP695*40)1Emcg
• Name: transgene insertion 1, Eileen McGowan
• MGI ID: MGI:3710691
• Synonyms: BRI-Abeta1-40, BRI-Abeta40
• Transgene: Tg(Prnp-ITM2B/APP695*40)1Emcg Location: unknown
• Alliance: Tg(Prnp-ITM2B/APP695*40)1Emcg page

Transgene origin

• Strain of Origin: (C3H x C57BL/6)F1 x C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The BRI-Abeta40 transgene was generated with a mouse prion promoter upstream of a BRI-Abeta40 fusion construct, containing a cDNA sequence from human type 2 transmembrane protein (BRI or ITM2B) fused in-frame with a "wildtype APP695" cDNA sequence encoding amyloid-beta40 (Abeta40) at the furin-like cleavage site; the C-terminal 23 amino acid ABri peptide of BRI was replaced with the Abeta40 sequence. A single founder line with the highest Abeta40 plasma levels (line 1d) was donated to The Jackson Laboratory. Transgenic fusion protein expression approximates levels of endogenous mouse APP expression and is expressed in patterns characteristic of the Prnp promoter, with highest expression in cerebellar granule cells and hippocampus. Full length Abeta fusion protein is most highly expressed with processed Abeta40 peptide expressed at lower levels. (J:101023)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:101023 McGowan E, et al., Abeta42 is essential for parenchymal and vascular amyloid deposition in mice. Neuron. 2005 Jul 21;47(2):191-9
• All: 5 reference(s)