Tg(Thy1-MAPT)22Schd
Transgene Detail

Summary

• Symbol: Tg(Thy1-MAPT)22Schd
• Name: transgene insertion 22, Katharina Schindowski
• MGI ID: MGI:3717232
• Synonyms: THY-Tau22
• Transgene: Tg(Thy1-MAPT)22Schd Location: unknown
• Alliance: Tg(Thy1-MAPT)22Schd page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-MAPT)22Schd expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MAPT (4137)

  Mapt	(MGI:97180)

• Mutation details: cDNA of the 412-amino acid 4-repeat isoform of the human MAPT gene was reamplified and modified by PCR-based site directed mutagenesis to introduce a Kozak-sequence and the mutations G272V and P301S (numbering according to longest human isoform). The cDNA was inserted into a Thy1.2 expression vector and the construct was microinjected into a C57BL/6 x CBA background. 10 copies of the transgene are detected in line 22 animals. Western blot analysis shows high levels of MAPT expression in the brains, and low levels in the spinal cords, of transgenic animals from line 22. (J:111982)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:111982 Schindowski K, et al., Alzheimer's disease-like tau neuropathology leads to memory deficits and loss of functional synapses in a novel mutated tau transgenic mouse without any motor deficits. Am J Pathol. 2006 Aug;169(2):599-616
• All: 30 reference(s)