Tg(PDGFB-APPSwInd)J9Lms
Transgene Detail

Summary

• Symbol: Tg(PDGFB-APPSwInd)J9Lms
• Name: transgene insertion J9, Lennart Mucke
• MGI ID: MGI:3717718
• Synonyms: APPSw,Ind, hAPPJ9, Tg(PDGF-APP*Swe*Ind)J9Lms, Tg(PDGFB-APP*)J9Lms
• Transgene: Tg(PDGFB-APPSwInd)J9Lms Location: unknown
• Alliance: Tg(PDGFB-APPSwInd)J9Lms page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(PDGFB-APPSwInd)J9Lms expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  The gene encodes for the Swedish and Indiana mutations.

• Mutation details: The transgene contains a human APP minigene encoding the Indiana mutation (V717F) which is associated with familial Alzheimer disease. The construct contained APP introns 6-8, allowing alternative splicing of exons 7 and 8. The human PDGF beta-chain promoter drives expression, and the construct also contains the SV40 polyadenylation signal. The Swedish APP (K670N/M671L) mutation was introduced into the construct by PCR primer modification. The modified construct was microinjected into (C57BL/6 x DBA/2)F2 one-cell embryos. Mice show high levels of amyloid beta deposits in their hippocampi. (J:23080, J:100974)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:100974 Hsia AY, et al., Plaque-independent disruption of neural circuits in Alzheimer's disease mouse models. Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3228-33
• All: 38 reference(s)