Tg(DMD-APP*)18Neve
Transgene Detail

Summary

• Symbol: Tg(DMD-APP*)18Neve
• Name: transgene insertion 18, Rachel L Neve
• MGI ID: MGI:3720650
• Synonyms: FLAGC100
• Transgene: Tg(DMD-APP*)18Neve Location: unknown
• Alliance: Tg(DMD-APP*)18Neve page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(DMD-APP*)18Neve expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  The gene encodes the C terminal 100 amino acids.

• Mutation details: A fragment consisting composed of the sequence ot the terminal 100 amino acids of the beta-amyloid precursor protein APP gene (C100, referred to as C104 in J:105050 which states that the transgene contains the terminal 100 amino acids of APP) was inserted into a plasmid encoding the neural-specific dystrophin (DMD) promoter. The vector also contained the SV40 early splice and polyadenylation sequences. A hydrophilic 10-amino acid FLAG sequence was engineered onto the aminoterminus of the C100 fragment. The construct was injected into (C57BL/6 x SJL)F2 fertilized eggs. Six founder mice were identified, 5 showing higher expression than the identified line 2. Line 18 was chosen to represent the higher expressing transgenic lines. RT-PCR revealed expression of the FLAGC100 in brains of all transgenic lines. (J:100978)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:100978 Neve RL, et al., Transgenic mice expressing APP-C100 in the brain. Neurobiol Aging. 1996 Mar-Apr;17(2):191-203
• All: 1 reference(s)