Tg(Ckm-APPSw)A2Lfa
Transgene Detail

Summary

• Symbol: Tg(Ckm-APPSw)A2Lfa
• Name: transgene insertion A2, Frank M LaFerla
• MGI ID: MGI:3720795
• Synonyms: A2 line, Tg(Ckm-APP*K670N*M671L)A2Lfa, Tg(Ckm-APP*Swe)A2Lfa
• Transgene: Tg(Ckm-APPSw)A2Lfa Location: unknown
• Alliance: Tg(Ckm-APPSw)A2Lfa page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ckm-APPSw)A2Lfa expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  The gene expresses the Swedish mutations.

• Mutation details: Human APP cDNA harboring the Swedish double mutation (K670N,M671L) was inserted downstream of the 1.3 kb 5' flanking sequence of the mouse muscle creatine kinase Ckm, upstream of the SV40 polyadenylation signal. The 3.8 kb construct was microinjected into pronuclei of single-cell C57Bl/6 x SJL embryos. Northern blot showed relatively low expression of the transgene in skeletal muscle of line A2 mice. (J:76338)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:76338 Sugarman MC, et al., Inclusion body myositis-like phenotype induced by transgenic overexpression of beta APP in skeletal muscle. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6334-9
• All: 1 reference(s)