Tg(Ckm-APPSw)A6Lfa
Transgene Detail

Summary

• Symbol: Tg(Ckm-APPSw)A6Lfa
• Name: transgene insertion A6, Frank M LaFerla
• MGI ID: MGI:3720796
• Synonyms: A6 line, Tg(Ckm-APP*K670N*M671L)A6Lfa, Tg(Ckm-APP*Swe)A6Lfa
• Transgene: Tg(Ckm-APPSw)A6Lfa Location: unknown
• Alliance: Tg(Ckm-APPSw)A6Lfa page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ckm-APPSw)A6Lfa expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  The gene expresses the Swedish mutations.

• Mutation details: Human APP cDNA harboring the Swedish double mutation (K670N,M671L) was inserted downstream of the 1.3 kb 5' flanking sequence of the mouse muscle creatine kinase Ckm, upstream of the SV40 polyadenylation signal. The 3.8 kb construct was microinjected into pronuclei of single-cell C57Bl/6 x SJL embryos. Northern blot showed relatively high expression of the transgene in skeletal muscle of line A6 mice, approximately 12 times higher than in Tg(Ckm-APP*K670N*M671L)A2Lfa mice. Western blot showed that transgenic protein expression is ~9 times higher than in mice from line A2. (J:76338)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:76338 Sugarman MC, et al., Inclusion body myositis-like phenotype induced by transgenic overexpression of beta APP in skeletal muscle. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6334-9
• All: 5 reference(s)