Tg(Th-SNCA*)1702Yosh
Transgene Detail

Summary

• Symbol: Tg(Th-SNCA*)1702Yosh
• Name: transgene insertion 1702, Makoto Yoshimoto
• MGI ID: MGI:3757756
• Synonyms: syn130m, Syn130m-1702
• Transgene: Tg(Th-SNCA*)1702Yosh Location: unknown Genetic Position: Chr8, cytoband B3
• Alliance: Tg(Th-SNCA*)1702Yosh page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Th-SNCA*)1702Yosh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SNCA (6622)

  Snca	(MGI:1277151)

• Tg(Th-SNCA*)1702Yosh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Th (25085)

  Th	(MGI:98735)

• Mutation details: The transgene construct contains the human alpha synuclein sequence with an A53T point mutation and a stop codon engineered at residue 130 which results in a truncated protein lacking the last 10 amino acids at the C-terminus. This sequence is under control of the rat tyrosine hydroxylase (Th) promoter (9 kb of 5' flanking region of the rat Th gene). The purified construct was injected into pronuclei of fertilized (C57BL/6 x C3H)F1 eggs. Copy number is estimated to be ~30. Expression of the transgene product is detected in the midbrain in soma and axons of neurons in the substantia nigra pars compacta. (J:125149)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:125149 Wakamatsu M, et al., Selective loss of nigral dopamine neurons induced by overexpression of truncated human alpha-synuclein in mice. Neurobiol Aging. 2008 Apr;29(4):574-85
• All: 3 reference(s)