Tg(Gfap-TNF*)K21Gkl
Transgene Detail

Summary

• Symbol: Tg(Gfap-TNF*)K21Gkl
• Name: transgene insertion K21, George Kollias
• MGI ID: MGI:3766134
• Synonyms: GFAP-tmTNF, TgK21
• Transgene: Tg(Gfap-TNF*)K21Gkl Location: unknown
• Alliance: Tg(Gfap-TNF*)K21Gkl page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gfap-TNF*)K21Gkl expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TNF (7124)

  Tnf	(MGI:104798)

• Mutation details: The -2663 to +93 region of the murine Gfap promoter, which has been shown to contain cis-regulatory elements required for astrocyte-specific expression was fused to human TNF genomic sequences containing a deletion mutation of the codons encoding the 1st to the 12th amino acids of the mature 17-kDa TNF protein. This mutant TNF gene has previously been shown to produce a transmembrane TNF protein that remains bioactive against cellular targets in vitro and in vivo. The genomic DNA was then ligated to the 0.77-kb EcoRI-Sal1 fragment containing the 3' UTR and polyadenylation site of the beta-globin gene, which replaces TNF 3' UTR sequences, thought to negatively regulate mRNA stability and translational efficiency. In this transgenic line, exogenous TNF protein production could be demonstrated by positive irnmunostaining of astrocytes in the spinal cord and brain sections using a polyclonal anti-human TNF Ab. (J:128072)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:128072 Akassoglou K, et al., Astrocyte-specific but not neuron-specific transmembrane TNF triggers inflammation and degeneration in the central nervous system of transgenic mice. J Immunol. 1997 Jan 1;158(1):438-45
• All: 5 reference(s)