Tg(Pfkl)224Yg
Transgene Detail

Summary

• Symbol: Tg(Pfkl)224Yg
• Name: transgene insertion 224, Yorum Groner
• MGI ID: MGI:3789607
• Synonyms: PFKL mini-gene
• Transgene: Tg(Pfkl)224Yg Location: unknown
• Alliance: Tg(Pfkl)224Yg page

Transgene origin

• Strain of Origin: (BALB/cBy x C57BL/6)F1 X (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pfkl)224Yg expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pfkl (MGI:97547)

• Mutation details: The transgenic construct for Tg(Pfkl)224Yg has 2.9 kb of the 5' genomic region, the first exon, first intron, and some of the second intron of Pfkl followed by the cDNA sequence extending to the 3' untranslated sequence and then genomic sequence from the 3' untranslated region was place downstream of this. A 22 base pair deletion was introduced into this 3' untranslated sequence in order to provide a system for detection of transgene-derived transcript. Line 224 was found to have several dozen copies of the transgene. (J:18425)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:18425 Elson A, et al., Overexpression of liver-type phosphofructokinase (PFKL) in transgenic-PFKL mice: implication for gene dosage in trisomy 21. Biochem J. 1994 Apr 15;299(Pt 2):409-15
• All: 1 reference(s)