Tg(Prnp-MAPT)7Vle
Transgene Detail

Summary

• Symbol: Tg(Prnp-MAPT)7Vle
• Name: transgene insertion 7, Virginia M Y Lee
• MGI ID: MGI:2385580
• Synonyms: T44
• Transgene: Tg(Prnp-MAPT)7Vle Location: unknown
• Alliance: Tg(Prnp-MAPT)7Vle page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp-MAPT)7Vle expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MAPT (4137)

  Mapt	(MGI:97180)

• Mutation details: A transgenic construct containing a cDNA sequence to human MAPT (fetal tau, Tau44 isoform) under the control of the mouse Prnp promtoer was used to create transgenic animals. Quantitative Western blot analysis showed hyperphosphorylated, insoluble MAPT protein to be widely expressed in neurons of the CNS at levels approximately 5 fold higher than the endogenous mouse counterpart. (J:61052)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Three founder lines were generated - lines 7, 27 and 43. Hemizygous mice from line 27 are not viable beyond 3 months and were not characterized in J:61052. Hemizygous mice from line 43 are phenotypically similar to line 7, however inclusions in the spinal cord are larger and more abundant than in line 7.

References

• Original: J:61052 Ishihara T, et al., Age-dependent emergence and progression of a tauopathy in transgenic mice overexpressing the shortest human tau isoform. Neuron. 1999 Nov;24(3):751-62
• All: 5 reference(s)