Tg(Itgax-TGFBR2)1Flv
Transgene Detail

Summary

• Symbol: Tg(Itgax-TGFBR2)1Flv
• Name: transgene insertion 1, Richard A Flavell
• MGI ID: MGI:3795208
• Synonyms: CD11c^dnR, Cd11c^dnTGFBR2, CD11c-dnTGFBRII
• Transgene: Tg(Itgax-TGFBR2)1Flv Location: unknown
• Alliance: Tg(Itgax-TGFBR2)1Flv page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Dominant negative, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Itgax-TGFBR2)1Flv expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TGFBR2 (7048)

  Tgfbr2

  (MGI:98729)

• Mutation details: The human TGFBRIII (Tgfbr2 transforming growth factor, beta receptor II) gene sequence between nucleotides -7 to +573 encoding the extracellular and transmembrane regions of TGFBR2 (dominant negative, dnTGFBR2) was inserted into exon 3 of the rabbit beta globin gene in the plasmid Itgax promoter vector pDOI-5. The construct was injected into (C57BL/6 x C3H)F1 hybrid fertilized eggs. RT-PCR confirmed expression of dnTGFBR2 in Itgax (CD11c)-expressing cells including subsets of natural killer cell (NK) and dendritic cell (DC) subsets. No expression is detected in NKT cells, T cells or B cells. Expression is sufficient to block TFG beta signaling through the functional receptor exclusively in DCs and NK cells. (J:98953)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:98953 Laouar Y, et al., Transforming growth factor-beta controls T helper type 1 cell development through regulation of natural killer cell interferon-gamma. Nat Immunol. 2005 Jun;6(6):600-7
• All: 14 reference(s)