Tg(HTT*97Q)IXwy
Transgene Detail

Summary

• Symbol: Tg(HTT*97Q)IXwy
• Name: transgene insertion I, X William Yang
• MGI ID: MGI:3800779
• Synonyms: BACHD, BACHD-I
• Transgene: Tg(HTT*97Q)IXwy Location: unknown
• Alliance: Tg(HTT*97Q)IXwy page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(HTT*97Q)IXwy expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HTT (3064)

  Htt	(MGI:96067)

• Mutation details: The 240 kb human bacterial artificial chromosome BAC(RP11-866L6), containing the entire 170 kb human Huntingtin (htt) genomic locus and approximately 20 kbp of 5' and 50 kbp of 3' flanking sequences, was modified by replacing the human htt exon 1 with a loxP-flanked human mutant htt exon 1 sequence (containing 97 mixed CAA-CAG repeats encoding a continuous polyglutamine (polyQ) stretch). DNA from a modified BAC clone was used as the source for the BACHD transgene and microinjected into FVB fertilized eggs. Mice from founder line BACHD-I were found to express relatively high levels of full-length human mutant Huntingtin (fl-mhtt) in the brain by qRT-PCR and the mice harbor tandem integrands of approximately 5 copies of the transgene. Another line, named BACHD-L, was analyzed, showing that the phenotypic aspects of line I were replicated in this strain. (J:137345)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:137345 Gray M, et al., Full-length human mutant huntingtin with a stable polyglutamine repeat can elicit progressive and selective neuropathogenesis in BACHD mice. J Neurosci. 2008 Jun 11;28(24):6182-95
• All: 82 reference(s)