Tg(H2-K-HLA-G,B2M)1Alm
Transgene Detail

Summary

• Symbol: Tg(H2-K-HLA-G,B2M)1Alm
• Name: transgene insertion 1, Andrew L Mellor
• MGI ID: MGI:3806366
• Synonyms: HLA-Gtg
• Transgene: Tg(H2-K-HLA-G,B2M)1Alm Location: unknown
• Alliance: Tg(H2-K-HLA-G,B2M)1Alm page

Transgene origin

• Strain of Origin: CBA/Ca

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(H2-K-HLA-G,B2M)1Alm expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  B2M (567)

  B2m	(MGI:88127)

  human

  HLA-G (3135)

  H2-D1	(MGI:95896)
  H2-K1	(MGI:95904)
  H2-M2	(MGI:95914)
  H2-M3	(MGI:95915)
  H2-M5	(MGI:95917)
  H2-M10.1	(MGI:1276522)
  H2-M10.2	(MGI:1276525)
  H2-M10.4	(MGI:1276527)
  H2-M10.6	(MGI:2687364)
  H2-M11	(MGI:2676637)
  H2-Q1	(MGI:95928)
  H2-Q2	(MGI:95931)
  H2-Q6	(MGI:95935)
  H2-Q7	(MGI:95936)
  H2-Q10	(MGI:95929)
  H2-T22	(MGI:95956)

• Mutation details: Two transgenic constructs were co-injected into male pronuclei of CBA/Ca zygotes. The constructs encoded (i) a H-2Kb/HLA-G transgene with the human HLA-G heavy chain (HLA-G; major histocompatibility complex, class I, G) gene inserted between the mouse H-2Kb MHC class I promoter and coding sequence, and (ii) a human B2M transgene (designed with the human beta2-microglobulin gene). (A third transgene was also co-injected as well, but was found not to have integrated into the genome.) The mean copy number of HLA-G genomic DNA per haploid mouse genome was 3 copies. HLA-G expression is observed in pre-implantation embryos and a variety of tissues; expression in the reproductive organs (uterus, ovary, and testes), lung, and liver is much more similar to mouse expression of Qa-2 than to Kb (despite the presence of the H-2Kb promoter and downstream coding sequence), while HLA-G expression in spleen, thymus and kidney correlates better with Kb expression patterns. (J:140011, J:140016)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:140011 Warner CM, et al., Symposium: innovative techniques in human embryo viability assessment. Soluble human leukocyte antigen-G and pregnancy success. Reprod Biomed Online. 2008 Oct;17(4):470-85
• All: 2 reference(s)