Tg(Gfap-GFAP*R239H)60TMIke
Transgene Detail

Summary

• Symbol: Tg(Gfap-GFAP*R239H)60TMIke
• Name: transgene insertion 60TM, Kazuhiro Ikenaka
• MGI ID: MGI:3809243
• Synonyms: 60TM
• Transgene: Tg(Gfap-GFAP*R239H)60TMIke Location: unknown
• Alliance: Tg(Gfap-GFAP*R239H)60TMIke page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gfap-GFAP*R239H)60TMIke expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GFAP (2670)

  Gfap	(MGI:95697)

• Mutation details: The original construct for this transgene consisted of a 2.5 kb fragment of the mouse Gfap promoter followed by a floxed eGFP cDNA, and a human GFAP alpha isophorm cDNA with the R239H mutation. Line A60 containing more than 10 copies of the construct was repeatedly crossed to a cre deleter strain to remove all of the copies of eGFP. The resulting line (60TM) lacks eGFP but retains an estimated 3 copies of the human GFAP cDNA. Each copy of the human GFAP is preceded by a loxP site allowing for Cre-mediated deletion of all but 1 copy of the human GFAP cDNA. (J:139349)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:139349 Tanaka KF, et al., Murine model of Alexander disease: analysis of GFAP aggregate formation and its pathological significance. Glia. 2007 Apr 15;55(6):617-31
• All: 5 reference(s)