Tg(Dspp-TGFB1)3Kul
Transgene Detail

Summary

• Symbol: Tg(Dspp-TGFB1)3Kul
• Name: transgene insertion 3, Ashok B Kulkarni
• MGI ID: MGI:3828146
• Synonyms: dTGF-beta1, Tg(TGFB1)3Kul
• Transgene: Tg(Dspp-TGFB1)3Kul Location: unknown
• Alliance: Tg(Dspp-TGFB1)3Kul page

Gross tooth abnormalities in Tg(Dspp-TGFB1)3Kul/? mice

Show the 3 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Dspp-TGFB1)3Kul expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  pig, domestic	TGFB1 (397078)

• Mutation details: The transgene comprises 6 kilobases of mouse genomic DNA containing the upstream regulatory elements of the dentin sialophosphoprotein (Dspp) gene, including the noncoding first exon and the first intron, followed by a 1.5 kb cDNA encoding a constitutively active porcine transforming growth factor beta 1, in which Cys223 and Cys225 have been replaced by serine, and an SV40 polyadenylation signal. Transgenic mouse lines with independent transgene integration sites were produced from 5 transgenic founder animals. This record represents the line of mice with a high level of transgene expression. TGFB1 is expressed from this transgene predominantly in the odontoblasts. (J:118918)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:118918 Thyagarajan T, et al., Reduced expression of dentin sialophosphoprotein is associated with dysplastic dentin in mice overexpressing transforming growth factor-beta 1 in teeth. J Biol Chem. 2001 Apr 6;276(14):11016-20
• All: 3 reference(s)