Tg(tetO-Ifng)184Pop
Transgene Detail

Summary

• Symbol: Tg(tetO-Ifng)184Pop
• Name: transgene insertion 184, Brian Popko
• MGI ID: MGI:4355871
• Synonyms: line 184 TRE/IFN-g, line 184 TRE/IFNg, line 184 TRE/IFN-gamma, TRE/IFN-gamma 184, TRE/IFN-gamma line 184
• Transgene: Tg(tetO-Ifng)184Pop Location: unknown
• Alliance: Tg(tetO-Ifng)184Pop page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Ifng)184Pop expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ifng (MGI:107656)

• Mutation details: The TRE/IFN-gamma transgene was designed with the PhCMV*-1 tetracycline-responsive promoter upstream of a mouse IFN-g cDNA sequence. The PhCMV*-1 tetracycline-responsive promoter contains the human cytomegalovirus (hCMV) minimal promoter fused to the tet-operator (tetO; TRE). The IFN-g cDNA is flanked by 40 bp of 5' and 57 bp of 3' UTR sequences and includes the translation start and stop signals. The sequences that confer IFN-g mRNA instability have been eliminated from the IFN-g cDNA sequence in an effort to increase steady-state mRNA levels derived from the transgene. This 2.3 kb transgene was microinjected into fertilized B6D2F1 oocytes. Founder line 184 was established. (J:94092)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:94092 Lin W, et al., Interferon-gamma induced medulloblastoma in the developing cerebellum. J Neurosci. 2004 Nov 10;24(45):10074-83
• All: 10 reference(s)