Tg(Vim*R113C)1Tmm
Transgene Detail

Summary

• Symbol: Tg(Vim*R113C)1Tmm
• Name: transgene insertion 1, Thomas M Magin
• MGI ID: MGI:4365636
• Synonyms: Vim R113C
• Transgene: Tg(Vim*R113C)1Tmm Location: unknown
• Alliance: Tg(Vim*R113C)1Tmm page

Transgene origin

• Strain of Origin: CBA

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Vim*R113C)1Tmm expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Vim (MGI:98932)	Transgene codes for a protein with an arginine to cysteine mutation.

• Mutation details: An 18 kb segment containing 10.5 kb of 5'- and 1 kb of 3'-regulatory sequence was isolated from a BALB/c cosmid library. The myc epitope was introduced by site-directed mutagenesis after serine at the second amino acid position.An additional mutation was introduced by site-directed mutagenesis that resulted in an arginine to cytosine change at amino acid residue 113. The transgenic mouse line was generated from a founder animal expressing the mutant at sub-stoichiometric level (<=30%) compared with the endogenous gene. (J:145782, J:153496)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:145782 Bornheim R, et al., A dominant vimentin mutant upregulates Hsp70 and the activity of the ubiquitin-proteasome system, and causes posterior cataracts in transgenic mice. J Cell Sci. 2008 Nov 15;121(Pt 22):3737-46
• All: 2 reference(s)