Et(EGFP/cre)16250Rdav
Transgene Detail

Summary

• Symbol: Et(EGFP/cre)16250Rdav
• Name: enhancer trap 16250, Ron Davis
• MGI ID: MGI:4947521
• Transgene: Et(EGFP/cre)16250Rdav Location: unknown
• Alliance: Et(EGFP/cre)16250Rdav page

Transgene origin

• Strain of Origin: C57BL/6J
• Project Collection: Neuroscience Blueprint cre

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Et(EGFP/cre)16250Rdav expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Et(EGFP/cre)16250Rdav (MGI:4947585)

• Mutation details: An enhancer trap lentiviral transgenic approach was used to generate these mice. A lentiviral vector transgene construct contained an EGFP/cre fusion gene under control of the mouse Thy1.2 minimal promoter (-270 to +30). The vector was designed with a self-inactivating lentiviral backbone (FUGW). The packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J embryos. Founder mice from line 16250 were crossed to B6-Gt(ROSA)26Sor^tm1Sor animals to assess cre activity/transmission. Cre positive mice were subsequently bred to C57BL/6 mice to generate a cre positive, reporter negative line. (J:150856)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Et(EGFP/cre)16250Rdav (mouse) Summary of all recombinase alleles driven by Et(EGFP/cre)16250Rdav. Comparative matrix view of recombinase activities Alliance mouse Et(EGFP/cre)16250Rdav gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009
• All: 2 reference(s)