Et(icre/ERT2)14208Rdav
Transgene Detail

Summary

• Symbol: Et(icre/ERT2)14208Rdav
• Name: enhancer trap 14208, Ron Davis
• MGI ID: MGI:4947569
• Transgene: Et(icre/ERT2)14208Rdav Location: unknown
• Alliance: Et(icre/ERT2)14208Rdav page

Transgene origin

• Strain of Origin: C57BL/6J
• Project Collection: Neuroscience Blueprint cre

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Et(icre/ERT2)14208Rdav expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Et(icre/ERT2)14208Rdav (MGI:4947622)

• Mutation details: An enhancer trap lentiviral transgenic approach was used to generate these mice. The transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a human synapsin minimal promoter (-244 to +47), a beta-globin intron, an icre /ERT2 fusion (icre; improved with mammalian codon usage, no putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), fused to a mutated human estrogen receptorligand binding domain (ERT2), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability. This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sor^tm1Sor mice to assess cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to create the 14208 line. (J:150856)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Et(icre/ERT2)14208Rdav (mouse) Summary of all recombinase alleles driven by Et(icre/ERT2)14208Rdav. Alliance mouse Et(icre/ERT2)14208Rdav gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009
• All: 1 reference(s)