Tg(tetO-TPR/MET,-EGFP)12Tcre
Transgene Detail

Summary

• Symbol: Tg(tetO-TPR/MET,-EGFP)12Tcre
• Name: transgene insertion 12, Tiziana Crepaldi
• MGI ID: MGI:4949465
• Synonyms: Tg(TPRMET-tetO-GFP)12Tcre, Tpr-Met-TetO-GFP, Tpr-Met-TRE-GFP
• Transgene: Tg(tetO-TPR/MET,-EGFP)12Tcre Location: unknown
• Alliance: Tg(tetO-TPR/MET,-EGFP)12Tcre page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A bi-directional tetracycline response element (TetO) drives expression of an enhanced green fluorescent protein reporter gene (EGFP) and of a cDNA encoding the human TRP-MET oncoprotein, in which the N-terminal of TPR (translocation promoter region), containing a pair of dimerization sites, is joined to the tyrosine kinase domain of the MET proto-oncogene, which is the receptor for hepatocyte growth factor. Crossing mice bearing this transgene with mice that express the tetracycline transactivator (tTA) or reverse transactivator (rtTA) protein under the control of a tissue-specific promoter permits regulation by tetracycline withdrawal/administration of the expression of TRP-MET and of the GFP reporter in the progeny. (J:120883)

Disease models

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Multiple founders whose fibroblasts expressed EGFP when infected with a tTA-expressing lentivirus under a ubiquitous promoter were used to establish mouse lines. Founder #12 has two independent insertions which segregate from one another. Sublines #12.2 and #12.9 were used in the reported studies. (J:120883)

References

• Original: J:120883 Crepaldi T, et al., Conditional activation of MET in differentiated skeletal muscle induces atrophy. J Biol Chem. 2007 Mar 2;282(9):6812-22
• All: 6 reference(s)