Tg(JPH3-GFP,-JPH3*)GXwy
Transgene Detail

Summary

• Symbol: Tg(JPH3-GFP,-JPH3*)GXwy
• Name: transgene insertion G, X William Yang
• MGI ID: MGI:5052365
• Synonyms: BAC-HDL2-STOP
• Transgene: Tg(JPH3-GFP,-JPH3*)GXwy Location: unknown
• Alliance: Tg(JPH3-GFP,-JPH3*)GXwy page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(JPH3-GFP,-JPH3*)GXwy expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  JPH3 (57338)

  Jph3	(MGI:1891497)

• Mutation details: The human BAC (RP11-33A21) was modified to include a transcriptional stop sequence (GFP followed by a floxed pgk-neo cassette and a polyA signal) before exon 1 and an expanded CTG/CAG track of 120 repeats in exon 2A of JPH3. RT-PCR confirmed that expression of the sense strand CUG repeat is silenced but expression of the antisense CAG repeat is retained. (J:174267)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:174267 Wilburn B, et al., An antisense CAG repeat transcript at JPH3 locus mediates expanded polyglutamine protein toxicity in Huntington's disease-like 2 mice. Neuron. 2011 May 12;70(3):427-40
• All: 1 reference(s)