Tg(Prnp-HTT*82Q)52Caro
Transgene Detail

Summary

• Symbol: Tg(Prnp-HTT*82Q)52Caro
• Name: transgene insertion 52, Christopher A Ross
• MGI ID: MGI:5304797
• Synonyms: N586-82Q
• Transgene: Tg(Prnp-HTT*82Q)52Caro Location: unknown
• Alliance: Tg(Prnp-HTT*82Q)52Caro page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp-HTT*82Q)52Caro expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HTT (3064)

  Htt	(MGI:96067)

• Mutation details: This transgene expresses the N-terminal 586 amino acids of human huntingtin (Htt) cDNA with a polyglutamine repeat length of 82, under the control of the mouse prion protein promoter. Amino acid 586 is the putative cleavage site of Htt by caspase 6 and so this transgene contains the caspase 6 fragment that would result from cleavage by caspase 6. This represents an intermediate-sized fragment. The mouse prion protein promoter drives transgene expression in the neurons of the central nervous system. Western blots show wide distribution of the protein in all brain regions tested. Immunoblots show that the N586-82Q fragment is cleaved into smaller fragments over time. Two lines were created, 52 and 91. Line 52 is a high-expressing line. (J:179364)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:179364 Waldron-Roby E, et al., Transgenic Mouse Model Expressing the Caspase 6 Fragment of Mutant Huntingtin. J Neurosci. 2012 Jan 4;32(1):183-193
• All: 1 reference(s)