Tg(NPHS2-Trpc6*E896K)F75aWalz
Transgene Detail

Summary

• Symbol: Tg(NPHS2-Trpc6*E896K)F75aWalz
• Name: transgene insertion F75a, Katherina Walz
• MGI ID: MGI:5319640
• Synonyms: Trpc6-HA E896K
• Transgene: Tg(NPHS2-Trpc6*E896K)F75aWalz Location: unknown
• Alliance: Tg(NPHS2-Trpc6*E896K)F75aWalz page

Transgene origin

• Strain of Origin: C57BL/6J x CBA/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(NPHS2-Trpc6*E896K)F75aWalz expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Trpc6 (MGI:109523)

• Mutation details: The Trpc6-HA wildtype cDNA sequence was made by modifying a Trpc6 cDNA sequence (encoding a 930 amino acid protein) originally isolated from mouse brain tissue by both repairing an A2615G point mutation (D872G) and also by in-frame addition of an influenza virus hemaglutinin epitope (HA-tag) at the 3' end. The cDNA sequence was placed downstream of a 2.5 kbp fragment from the promoter/enhancer region of the human podocin locus (NPHS2) and upstream from a bovine growth hormone polyA sequence. The G2686A nucleotide substitution was introduced to produce the amino acid substitution of lysine for glutamic acid at position 896 (E896K). Lines F73a and F75a were generated. (J:165112)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:165112 Krall P, et al., Podocyte-specific overexpression of wild type or mutant trpc6 in mice is sufficient to cause glomerular disease. PLoS One. 2010;5(9):e12859
• All: 1 reference(s)