Tg(Prnp*)#Rgab
Transgene Detail

Summary

• Symbol: Tg(Prnp*)#Rgab
• Name: transgene insertion, Ruth Gabizon
• MGI ID: MGI:5428331
• Synonyms: TgMHu2ME199K, (TgMHu2M) E199KPrP, TgMHu2ME200K
• Transgene: Tg(Prnp*)#Rgab Location: unknown
• Alliance: Tg(Prnp*)#Rgab page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp*)#Rgab expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: The Syrian hamster promoter drives expression of the chimeric mouse/human cDNA MHu2M containing nucleotide substitutions that results in the amino acid substitutions of methionine for leucine at position 108, methionine for valine at position 111, methionine for isoleucine at position 138, asparagine for tyrosine at position 154, asparagine for serine at position 169 with the additional substitution of lysine for glutamic acid at position 199. This mutation corresponds to the human mutation E200K associated with Creutzfeldt-Jakob disease. Two lines were generated. The pound symbol (#) is used when no line is specified or lines or pooled. (J:183170)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This transgene was originally generated in oocytes containing Prnp^tm1Cwe.

References

• Original: J:183170 Friedman-Levi Y, et al., Fatal prion disease in a mouse model of genetic E200K Creutzfeldt-Jakob disease. PLoS Pathog. 2011 Nov;7(11):e1002350
• All: 9 reference(s)