Tg(Pklr-Myc)73Ak
Transgene Detail

Summary

• Symbol: Tg(Pklr-Myc)73Ak
• Name: transgene insertion 73, Axel Kahn
• MGI ID: MGI:5428913
• Synonyms: L-PK/c-myc73
• Transgene: Tg(Pklr-Myc)73Ak Location: unknown
• Alliance: Tg(Pklr-Myc)73Ak page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pklr-Myc)73Ak expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Myc (MGI:97250)

• Mutation details: This transgene consists of a 2.4 kb fragment containing exons 2 and 3 of the murine c-myc oncogene under the control of the rat L-type pyruvate kinase gene regulatory region. The 3' UTR of the Myc gene was replaced with the 3' UTR region of SV40 early gene to enhance stability of myc mRNA. Transcription of Pklr is dependent on metabolic and hormonal controls, such that glucose and insulin increase transcription, while fasting, refeeding a low-carbohydrate diet and glucagon inhibit Pklr transcription. Thus manipulation of diet can result in expression of the transgene. Line 73 carries approximately 100 copies of the transgene. Northern blot analysis indicates transgene expression in the liver and to a lesser degree in the small intestine and kidney. (J:185488)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:185488 Cartier N, et al., Diet-dependent carcinogenesis of pancreatic islets and liver in transgenic mice expressing oncogenes under the control of the L-type pyruvate kinase gene promoter. Oncogene. 1992 Jul;7(7):1413-22
• All: 3 reference(s)