Tg(NES/TK-PDGFB,-lacZ)310Kfn
Transgene Detail

Summary

• Symbol: Tg(NES/TK-PDGFB,-lacZ)310Kfn
• Name: transgene insertion 310, Karin Forsberg-Nilsson
• MGI ID: MGI:5448202
• Synonyms: nes/tk-Pdgfb-lacZ
• Transgene: Tg(NES/TK-PDGFB,-lacZ)310Kfn Location: unknown
• Alliance: Tg(NES/TK-PDGFB,-lacZ)310Kfn page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(NES/TK-PDGFB,-lacZ)310Kfn expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PDGFB (5155)

  Pdgfb

  (MGI:97528)

• Mutation details: This transgene consists of the human nestin second intron enhancer joined to a basic thymidine kinase promoter and the human PDGFB cDNA (without the 5' and 3' untranslated regions) followed by an internal ribosomal entry site (IRES) and a lacZ reporter gene. The minimal nestin enhancer element is specific for embryonic expression but will not drive adult expression of PDGF-B. Three lines were made: 310, 312, and 319. Beta-galactosidase assays show that the transgene is expressed in the developing CNS, with high expression at E10.5, limited expression at E17.5 and no expression at P0. In the retina, transgene expression commences on E17.5 and attains maximal level between P1 and P15, before declining on P20. (J:165035, J:189829)

Disease models

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:165035 Niklasson M, et al., Enlarged lateral ventricles and aberrant behavior in mice overexpressing PDGF-B in embryonic neural stem cells. Exp Cell Res. 2010 Oct 15;316(17):2779-89
• All: 2 reference(s)