Tg(Myh6-Tpm1*D175N)#Dfw
Transgene Detail

Summary

• Symbol: Tg(Myh6-Tpm1*D175N)#Dfw
• Name: transgene insertion, David F Wieczorek
• MGI ID: MGI:5462246
• Synonyms: alpha-TM 175
• Transgene: Tg(Myh6-Tpm1*D175N)#Dfw Location: unknown
• Alliance: Tg(Myh6-Tpm1*D175N)#Dfw page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Tpm1*D175N)#Dfw expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tpm1 (MGI:98809)	Transgene codes for a protein with an aspartic acid to asparagine mutation.

• Mutation details: alpha-Tpm1 cDNA was subjected to site-directed mutagenesis to generate a GAC to AAC transition at codon 175, which is predicted to result in an Asp to Asn amino acid change in the encoded protein. The mutated cDNA was then inserted downstream of the alpha-MHC promoter, which confers cardiac-specific expression. Transgenic lines 69, 108, 109, and 113, were produced and were selected for additional studies. (J:57365)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:57365 Muthuchamy M, et al., Mouse model of a familial hypertrophic cardiomyopathy mutation in alpha-tropomyosin manifests cardiac dysfunction. Circ Res. 1999 Jul 9;85(1):47-56
• All: 5 reference(s)