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Tg(Thy1-APPArc)M8Cagr
Transgene Detail
Summary
Symbol: Tg(Thy1-APPArc)M8Cagr
Name: transgene insertion M8, Caroline Graff
MGI ID: MGI:5463435
Synonyms: TgAPParc, Thy1.2-hAPParc
Transgene: Tg(Thy1-APPArc)M8Cagr  Location: unknown  
Alliance: Tg(Thy1-APPArc)M8Cagr page
Transgene
origin
Strain of Origin:  C57BL/6 x CBA
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Thy1-APPArc)M8Cagr expresses 1 gene
 
Mutation detailsThe Arctic mutation, an A>G nucleotide substitution resulting in a glutamic acid to glycine amino acid substitution at codon 693 (E693G), was introduced into a human cDNA of APP (695 isoform) by site-directed mutagenesis and a Kozak sequence was introduced upstream of ATG to enhance translation. This construct was placed under the control of the mouse Thy1.2 expression cassette that drives expression of the transgene specifically in neurons. Two lines were made, M7 and M8. M8 is the high expressor line and hemizygous mice have an approximately 3-fold higher expression of human APP compared to endogenous App. (J:168288)
Phenotypes
Key:
hm homozygous ht heterozygous tg involves transgenes phenotype observed
cn conditional genotype  cx complex: > 1 genome feature ot other: hemizygous, indeterminate,... N normal phenotype
Genotype/
Background:
Allelic Composition
Genetic Background
Cell Line(s)
tg1  Disease Model
involves: C57BL/6 * CBA
 
tg2  Disease Model
involves: C57BL/6 * CBA
 
Phenotypes:
Affected Systems
show or hide all annotated terms Sex:
 
behavior/neurological
behavior/neurological phenotype
N N
abnormal spatial learning
abnormal long-term spatial reference memory
abnormal spatial working memory
homeostasis/metabolism
amyloid beta deposits
nervous system
amyloid beta deposits
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Key:
disease model   expected model not found
Models:
Human Diseases
tg1
tg2
IDs
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:168288 Ronnback A, et al., Progressive neuropathology and cognitive decline in a single Arctic APP transgenic mouse model. Neurobiol Aging. 2011 Feb;32(2):280-92
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory