Tg(CAG-Diaph3)771Lesp
Transgene Detail

Summary

• Symbol: Tg(CAG-Diaph3)771Lesp
• Name: transgene insertion 771, Marci Lesperance
• MGI ID: MGI:5468143
• Synonyms: Tg(CAG-Diap3)771Lesp
• Transgene: Tg(CAG-Diaph3)771Lesp Location: unknown
• Alliance: Tg(CAG-Diaph3)771Lesp page

The stereocilia of Tg(CAG-Diaph3)771Lesp/0 and Tg(CAG-Diap3)924Lesp/0 mice become progressively abnormal from 4 to 24 weeks of age

Show the 3 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-Diaph3)771Lesp expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Diaph3 (MGI:1927222)

• Mutation details: The insertion vector was approximately 7 kb, containing the CAG promoter and the Diaph3 complete coding sequence. Genotyping by PCR revealed that 10 of 83 potential transgenic founders had integrated the transgene. Five founders transmitted the transgene with offspring born in expected Mendelian ratios. Lines 771 and 924 exhibited hearing loss within the first 16 weeks of life in the F1 generation as detected by auditory brainstem response (ABR) testing and were selected for further analysis. (J:193237)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:193237 Schoen CJ, et al., Diaphanous homolog 3 (Diap3) Overexpression Causes Progressive Hearing Loss and Inner Hair Cell Defects in a Transgenic Mouse Model of Human Deafness. PLoS One. 2013;8(2):e56520
• All: 2 reference(s)