Tg(Thy1-Tcf4)1Mjro
Transgene Detail

Summary

• Symbol: Tg(Thy1-Tcf4)1Mjro
• Name: transgene insertion 1, Moritz J Rossner
• MGI ID: MGI:5521185
• Synonyms: Tcf4tg, Tg[Thy-1-Tcf4]1Mr
• Transgene: Tg(Thy1-Tcf4)1Mjro Location: unknown
• Alliance: Tg(Thy1-Tcf4)1Mjro page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-Tcf4)1Mjro expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcf4 (MGI:98506)

• Mutation details: This transgene consists of the full-length mouse Tcf4 open reading frame under the control of the Thy1.2 minigene, which restricts expression to the postnatal brain. In addition a Flag-tag was added on the N-terminus and a TAP-tag on the C-terminus of Tcf4. qRT-PCR shows approximately 1.5-fold overexpression in the hippocampus and expression in the cortex and amygdala but not in the striatum. Western blotting confirmed protein expression in the cortex and hippocampus but not in the cerebellum and in situ hybridization shows expression in the hippocampus, the neocortex, thalamus, and mesencephalic structures of adults. Western blotting also shows that transgene expression first occurs at postnatal day 2. (J:202664)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:202664 Brzozka MM, et al., Cognitive and sensorimotor gating impairments in transgenic mice overexpressing the schizophrenia susceptibility gene Tcf4 in the brain. Biol Psychiatry. 2010 Jul 1;68(1):33-40
• All: 3 reference(s)