Tg(HBB-lacZ)ALey
Transgene Detail

Summary

• Symbol: Tg(HBB-lacZ)ALey
• Name: transgene insertion A, Timothy Ley
• MGI ID: MGI:5524131
• Synonyms: Beta-globin-lacZ, Beta-lacZ tg
• Transgene: Tg(HBB-lacZ)ALey Location: unknown Genetic Position: Chr4, Syntenic
• Alliance: Tg(HBB-lacZ)ALey page

Transgene origin

• Strain of Origin: C57BL/6NTac

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A human beta-globin locus control region HS2 (DNaseI hypersensitive site 2) fragment was linked upstream in tandem to an HBB transgene that has a lacZ cassette in place of the region from the initiation codon to the BamH1 site of the HBB gene sequence. Regulatory sequences of HBB are retained in the 5' flanking region, in IVS-2 (second intervening sequence), and 3' to the gene. 10 founder lines were produced, and line A (copy number of 57) which has high beta-galactosidase activity in the periperal blood. The ES cell line generated from this transgene carries the transgene on distal chromosome 4. (J:203044)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:203044 Graubert TA, et al., Stochastic, stage-specific mechanisms account for the variegation of a human globin transgene. Nucleic Acids Res. 1998 Jun 15;26(12):2849-58
• All: 1 reference(s)